GSM4061726: HCC827_AKT1; Homo sapiens; RNA-Seq - SRA

SRX6804715: GSM4061726: HCC827_AKT1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 40.2M spots, 12.1G bases, 3.5Gb downloads

Submitted by: NCBI (GEO)

Study: Strand specific Sequencing of myr-Akt1 induced transcriptomes in NSCLC cell lines

PRJNA564014 • SRP220432 • All experiments • All runs

show Abstracthide Abstract

To identify the genes significantly regulated by Akt1 activation in NSCLC, myr-Akt1 stable over-expressed A549, HCC827 cells and control vector cells were constructed. The transcriptomes were analyzed by rRNA-depleted strand specific RNA-seq, and LINC01546 was identified significantly up-regulated in myr-Akt1 A549 and HCC827 cells, as compared to vector cells. Overall design: Transcriptomes profiles of A549-myr-Akt1, A549-vector, HCC827-myr-Akt1 and HCC827-vecotr were generated by Illumina NovaSeq 6000.

Sample: HCC827_AKT1

SAMN12698164 • SRS5349633 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was harvested using Trizol reagent. RNA was prepared by using Ribo-Zero Magnetic Kit (rRNA-depleted RNA). Illumina TruSeq Stranded mRNA Kit was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM4061726

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 40.2M spots, 12.1G bases, 3.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR10071128	40,232,287	12.1G	3.5Gb	2019-11-01

ID:: 8963304

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity